Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma

<div>Representative images of hematoxylin/eosin staining of all the experimental groups (negative control (only skin) with no specific day and H<sub>2</sub>O, H<sub>2</sub>O + EP, pControl + EP, LPS (positive control), and pORF-mIL-12-ORT + EP) on day 1, day 4, day 8, and day 14 (11). A semiquantitative scoring system for immunopositive cells was used: (+) low, (++) moderate, and (+++) high positivity. Various histological characteristics of the figures are marked: immune cell infiltration (<b>→</b>), blood vessels (▲), melanin (<b>→</b>), necrosis (●), and epithelium damage (◊). The images were taken under 20x magnification (numerical aperture 0.85).</div>

Mediators of Inflammation

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Figure 3: Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma 

Figure 3 | Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma 