Mediators of Inflammation / 2017 / Article / Tab 1

Review Article

Paracoccidioides Spp.: Virulence Factors and Immune-Evasion Strategies

Table 1

Relative content of the main polysaccharides present in the yeast cell wall of different strains of Paracoccidioides brasiliensis. Strains belonging to at least three different cryptic species were grown at 37°C on RPMI 1640 (Gibco) liquid medium, buffered with 0.165 M morpholinepropanesulfonic acid (MOPS) to pH 7.0 for 4 days.

Morphological phasePolysaccharidePolysaccharide content per P. brasiliensis strain (cryptic species)
Pb73 (PS3)Pb300 (PS4)Pb377 (PS4)Pb444 (PS4)Pb381 (S1)

Mα-(1,3)-Glucan1.06 ± 0.5trtrtr7.0 ± 0.3
β-(1,3)-Glucan31.4 ± 0.425.4 ± 0.127.7 ± 0.220.2 ± 1.322.2 ± 1.1
Chitin13.2 ± 0.717.3 ± 0.412.6 ± 0.68.6 ± 0.213.5 ± 0.5

Yα-(1,3)-Glucan22.4 ± 0.923.7 ± 0.223.8 ± 0.424.1 ± 0.832.6 ± 1.0
β-(1,3)-Glucan10.6 ± 0.66.8 ± 0.53.9 ± 0.28.6 ± 0.46.3 ± 0.3
Chitin35.1 ± 1.331.4 ± 0.618.0 ± 0.226.6 ± 0.823.5 ± 0.8

tr stands for traces.

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