Innate Immunity of Adipose Tissue in Rodent Models of Local and Systemic Staphylococcus aureus Infection
Table 2
Gene expression levels in adipose tissue from S. aureus infected peritonitis animals.
Genes
Model
Adipose tissue
Fold-expression ΔΔCt
p
n
Inflammation
PYCARD
Mouse, 24 h
Epididymal
0.0002
6
PYCARD
Mouse, 48 h
Epididymal
0.0001
5
PYCARD
Mouse, 24 h
Subcut.
0.0183
6
PYCARD
Mouse, 48 h
Subcut.
0.0434
5
Interleukin-6
Mouse, 24 h
Epididymal
0.0025
6
Interleukin-6
Mouse, 48 h
Epididymal
0.0001
5
Interleukin-6
Mouse, 24 h
Subcut.
0.0310
6
Interleukin-6
Mouse, 48 h
Subcut.
0.0118
5
MCP-1
Mouse, 24 h
Epididymal
0.0003
6
MCP-1
Mouse, 48 h
Epididymal
0.0001
5
MCP-1
Mouse, 24 h
Subcut.
0.0893
6
MCP-1
Mouse, 48 h
Subcut.
0.1340
5
TLR2
Mouse, 24 h
Epididymal
0.054
7-8
TLR2
Mouse, 48 h
Epididymal
0.315
4–7
TLR2
Mouse, 24 h
Subcut.
0.686
4
Cathelicidin
Mouse, 24 h
Epididymal
0.798
8
Cathelicidin
Mouse, 48 h
Epididymal
0.933
4–8
Cathelicidin
Mouse, 24 h
Subcut.
0.343
4
Lipocalin-2
Mouse, 24 h
Epididymal
0.001
6–8
Lipocalin-2
Mouse, 48 h
Epididymal
0.010
4–6
Lipocalin-2
Mouse, 24 h
Subcut.
0.029
4
Adipokines
Adiponectin
Mouse, 24 h
Epididymal
0.2870
6
Adiponectin
Mouse, 48 h
Epididymal
0.4830
5
AdipoR1
Mouse, 24 h
Epididymal
0.2193
6
AdipoR1
Mouse, 48 h
Epididymal
0.3466
5
Visfatin
Mouse, 24 h
Epididymal
0.7833
6
Visfatin
Mouse, 48 h
Epididymal
0.7944
5
Resistin
Mouse, 24 h
Epididymal
0.0166
6
Resistin
Mouse, 48 h
Epididymal
0.0451
5
Resistin
Mouse, 24 h
Subcut.
0.9895
6
Resistin
Mouse, 48 h
Subcut.
0.0688
5
Leptin
Mouse, 24 h
Epididymal
0.007
15–17
Leptin
Mouse, 48 h
Epididymal
0.005
13–15
Leptin
Mouse, 24 h
Subcut.
0.057
4
Browning
CD137
Mouse, 24 h
Epididymal
0.050
8
CD137
Mouse, 48 h
Epididymal
0.004
4–8
CD137
Mouse, 24 h
Subcut.
0.886
4
UCP-1
Mouse, 24 h
Epididymal
0.886
4
UCP-1
Mouse, 24 h
Subcut.
0.114
4
Lipids/metabolism
LOX-1
Mouse, 24 h
Epididymal
0.0023
6
LOX-1
Mouse, 48 h
Epididymal
0.0004
5
LOX-1
Mouse, 24 h
Subcut.
0.0110
6
LOX-1
Mouse, 48 h
Subcut.
0.0002
5
PPARγ
Mouse, 24 h
Epididymal
0.035
17-18
PPARγ
Mouse, 48 h
Epididymal
<0.001
13–17
PPARγ
Mouse, 24 h
Subcut.
0.343
4
The expression of selected genes in epididymal and subcutaneous adipose tissue obtained from mice with S. aureus peritonitis was analyzed and compared to adipose tissue from uninfected control animals. This table depicts the gene expression difference (ΔΔCt) versus uninfected control animals for genes with a ΔΔCt of at least 1. MCP-1, monocyte chemoattractant protein-1; PYCARD, PYD and CARD domain-containing; AdipoR1, adiponectin receptor type-1; CD137, tumor necrosis factor receptor superfamily, member 9; LOX-1, lectin-like oxidized-LDL receptor-1; Ptpn-1, protein tyrosine phosphatase-1; UCP-1, uncoupling protein-1; PPARγ, peroxisome proliferator-activated receptor-γ; subcut., subcutaneous adipose tissue. : no change; : upregulation; : downregulation.
