Key Role of STAT4 Deficiency in the Hematopoietic Compartment in Insulin Resistance and Adipose Tissue Inflammation

Figure 6

Insulin signaling in chimeric mice following bone marrow transplant and in Rag1−/− mice following adoptive transfer and HFD. (a) Chimeric mice following BMT. STAT4−/− and STAT4+/+ recipient mice (/group) were injected i.p. with a 10 U/kg insulin bolus and tissues were harvested after 10 minutes. Western blot analysis showed increased activation of the insulin receptor (pIR) in liver and insulin receptor substrate-1 (pIRS-1) in muscle of the STAT4−/− recipient mice; no differences were found between the two groups in epididymal adipose tissue. Data represents mean ± SD; . ((b) and (c)) Rag1−/− mice following adoptive transfer. Adipocytes (b) were isolated from epididymal adipose tissue of different mice groups and stimulated in vitro with 5 nM insulin, for 30 min. Insulin receptor-1 and PKC (Akt) activation were measured by western blot (-4/group). (c) Mice were injected with an insulin bolus (10 U/kg) and euthanized after 10 minutes. Adipose tissue was harvested and processed immediately for western blot analysis. Results are representative for 2-3 mice/group.

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