Different Dietary Proportions of Fish Oil Regulate Inflammatory Factors but Do Not Change Intestinal Tight Junction ZO-1 Expression in Ethanol-Fed Rats

Figure 4

IHC staining of the tight junction protein, ZO-1, in the small-intestinal mucosa in each group. C: control group; CF25: control diet with fish oil substituted for 25% of olive oil; CF57: control diet with fish oil substituted for 57% of olive oil; E: ethanol group; EF25: alcohol-containing diet with fish oil substituted for 25% of olive oil; EF57: alcohol-containing diet with fish oil substituted for 57% of olive oil. (a) Representative histological images of rats in all groups at 200x magnification. Arrows indicate ZO-1-positive areas. The normal small intestine exhibited intact epithelium with marked dark-brown ZO-1 expression. (b) Quantification of ZO-1-immunoreactive areas among groups. Bars with different letters (A, B) significantly differ among groups C, CF25, and CF57 at the level according to a one-way analysis of variance (ANOVA) followed by Duncan’s new multiple range test.

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