Research Article
A Novel Role for Brain Natriuretic Peptide: Inhibition of IL-1 Secretion via Downregulation of NF-kB/Erk 1/2 and NALP3/ASC/Caspase-1 Activation in Human THP-1 Monocyte
Figure 6
BNP is involved all the molecular mechanisms controlling IL-1β production and release by inhibiting the activation of NF-kB, ERK 1/2 and of all the elements of NALP3 inflammasome/ASC/Caspase-1 cascade in macrophage-like cells. PMA differentiated THP-1 cells were treated with 10−8 M BNP in absence or presence of 10 μg/mL LPS/5 mM ATP for 30 min. Cell lysates were immunoblotted for P-IkB-α and P-ERK1/2 (a) or NALP3, ASC, or Caspase-1 (b). The blots were stripped of the bound Ab and reprobed with mouse anti--actin, to confirm equal loading. Western blots are representative of three separate experiments.
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