Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation

Figure 4

U251-NF-κB-GFP-Luc cells are suitable for testing the anti-inflammatory potential of drugs (a). The NF-κB-inhibitor Bay11-7082 significantly reduced NF-κB-dependent luciferase activity at concentrations higher than 1.5 μM. Vehicle: DMSO. (b) The IKKβ inhibitor IMD0354 diminished NF-κB-dependent luciferase activity at various concentrations (0.1, 1, and 10 μM) both in the presence or absence of 1 μg/ml E. coli LPS. Data are presented as mean ± SEM from at least 3 independent experiments. , , and were considered significant, ANOVA with Bonferroni correction, CI 95%.

(a)

(b)