Intranasal Delivery of miR-146a Mimics Delayed Seizure Onset in the Lithium-Pilocarpine Mouse Model

Figure 5

Protective effects of miR-146a mimics on the hippocampus 2 h after pilocarpine administration. (a) Representative HE-stained slices showed that the hippocampal nuclei (blue) almost disappeared in the APE group but were markedly stained in the APT group; representative TUNEL- and DAPI-stained slices showed all hippocampal cells (DAPI in blue) and significant apoptosis of hippocampal cells (TUNEL in green, merged TUNEL and DAPI in cyan) in the APE group, but no visible apoptosis was observed in the APT group. (b) To prevent manual mistakes, the apoptotic indices were calculated using Image-Pro plus 6.0 (Media Cybernetics, Bethesda, USA), which were consistently lower in all regions of the hippocampus (CA1, CA2, CA3, and DG) of the APT group than the APE group..

(a)

(b)