Metformin Suppressed CXCL8 Expression and Cell Migration in HEK293/TLR4 Cell Line

Figure 1

Metformin inhibited LPS-induced CXCL8 expression. (a) Metformin cytotoxicity. HEK294/TLR4/TLR4 cells were treated with serial concentrations of metformin for 24 h. Cytotoxicity was measured through using MTT assay. Data are reported as mean ± SEM. Statistically significant differences are indicated by an asterisk (, compared to control). (b) LPS-induced CXCL8 expression. HEK294/TLR4 cells were treated with serial LPS concentrations for 24 hours. CXCL8 concentration in the culture media was then measured by ELISA kit. Data are reported as mean ± SEM. (c, d) LPS-induced CXCL8 expression was suppressed by metformin pretreatment for 24 h (c) or 48 h (d). HEK294/TLR4 cells were treated with different concentrations of metformin for 24 or 48 h, followed by 24 h incubation with LPS. CXCL8 concentration in the culture media was measured via using ELISA kit. Data are normalized by CXCL8 concentration of cells treated with LPS only. , , and compared to 1 μg/ml LPS group.

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