Anti-Inflammatory Strategy for M2 Microglial Polarization Using Retinoic Acid-Loaded Nanoparticles

Figure 1

RA-NP did not compromise microglia cell viability. (a) Microglial cells were treated with RA-NP and blank NP (24 hours) to assess toxicity, using MTT assay. Cytotoxicity was induced at concentrations starting at 100 μg/mL (; and compared to untreated cells). (b) LPS (100 ng/mL) did not potentiate cytotoxicity; only blank nanoparticles (100 μg/mL) in the presence of LPS significantly compromised viability (; compared to untreated cells). (c) RA-NP (30 μg/mL) internalization by microglial cells was observed by confocal microscopy over the course of 24 hours, in the absence (top row) or presence of LPS (bottom row). Scale bar 10 μm.

(a)

(b)

(c)