Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4⁺CD25⁺ Regulatory T Cells Mainly through Axl Receptor

Figure 2

Immunofluorescence detection of TAM receptors. CD4⁺CD25⁺Tregs were incubated with anti-Axl (10 μg/ml) and anti-Mertk (10 μg/ml) monoclonal Ab, respectively, followed by FITC-labeled goat anti-rat IgG as the secondary Ab (1 : 30). The cells were analyzed by fluorescent confocal microscopy. Representative photomicrographs show that FITC-positive cells (green) were detected among PE-positive CD4⁺CD25⁺Tregs (red). Those double-stained cells are shown in yellow.