Growth Arrest-Specific 6 Enhances the Suppressive Function of CD4⁺CD25⁺ Regulatory T Cells Mainly through Axl Receptor

Figure 7

Gas6 modulates the suppressive function of Tregs mainly through Axl receptor. Axl knockdown Tregs were pretreated with 100 ng/ml rmGas6 for 24 h and then mixed with CD4⁺CD25⁻T cells in presence of anti-CD3/CD28 Abs. After 72 h, the suppressive function of CD4⁺CD25⁺T cells was determined by CFSE assay (a and b) (/group). (A) CD4⁺CD25⁻T cells alone. (B) CD4⁺CD25⁻T cells were stimulated with a combination of soluble anti-CD3 and anti-CD28 antibodies. (C) CD4⁺CD25⁺Tregs and (D) rmGas6-pretreated Tregs were mixed with CFSE-labeled CD4⁺CD25⁻T cells at a ratio of 1 : 1 in the presence of anti-CD3 and anti-CD28 antibodies. (E) siRNA-control and (F) siRNA-Axl transfected CD4⁺CD25⁺Tregs pretreated with Gas6 were cocultured with CFSE-labeled CD4⁺CD25⁻T cells. .

(a)

(b)