Table of Contents Author Guidelines Submit a Manuscript
Mediators of Inflammation
Volume 2017 (2017), Article ID 6898505, 9 pages
https://doi.org/10.1155/2017/6898505
Research Article

Morita-Baylis-Hillman Adducts Display Anti-Inflammatory Effects by Modulating Inflammatory Mediator Expression in RAW264.7 Cells

1Laboratório de Biotecnologia Celular e Molecular, Departamento de Biotecnologia, Centro de Biotecnologia, Universidade Federal da Paraíba, Campus I, João Pessoa, PB, Brazil
2Laboratório de Imunofarmacologia, Departamento de Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal da Paraíba, Campus I, João Pessoa, PB, Brazil
3Laboratório de Síntese Orgânica Medicinal da Paraíba (LASOM-PB), Departamento de Química, Universidade Federal da Paraíba, Campus I, João Pessoa, PB, Brazil

Correspondence should be addressed to Glaucia V. Faheina-Martins; rb.moc.oohay@aniehafaicualg and Demetrius A. M. Ara├║jo; rb.bpfu.cetoibc@suirtemed

Received 27 January 2017; Revised 19 May 2017; Accepted 5 June 2017; Published 12 July 2017

Academic Editor: Manoj K. Mishra

Copyright © 2017 Glaucia V. Faheina-Martins et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Inflammatory response plays an important role not only in the normal physiology but also in pathologies such as cancers. The Morita-Baylis-Hillman adducts (MBHA) are a novel group of synthetic molecules that have demonstrated many biological activities against some parasitic cells such as Plasmodium falciparum, Leishmania amazonensis, and Leishmania chagasi, and antimitotic activity against sea urchin embryonic cells was also related. However, little is known about the mechanisms induced by MBHA in inflammatory process and its relation with anticancer activity. The present work investigated the cytotoxicity of three MBHA derivatives (A2CN, A3CN, and A4CN), on human colorectal adenocarcinoma, HT-29 cells, and their anti-inflammatory activities were examined in lipopolysaccharide- (LPS-) stimulated RAW264.7 macrophage cells, being these derivatives potentially cytotoxic to HT-29 cells. Coincubation with A2CN, A3CN, or A4CN and LPS in RAW264.7 cells inhibited NO production, as well as the production of reactive oxygen species (ROS) was also repressed. The mRNA expressions of IL-1β and IL-6 were significantly downregulated by such MBHA compounds in RAW264.7 cells, but only A2CN was able to inhibit the COX-2 gene expression. We also showed that MBHA compounds decreased almost to zero the production of IL-1β and IL-6. These findings display that such MBHA compounds exhibit anticancer and anti-inflammatory activities.