Mediators of Inflammation / 2017 / Article / Fig 1

Research Article

Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells

Figure 1

Expression levels of USP2 and IL6 in macrophages following TLR ligand stimulation. (a) HL-60 cells were pretreated with PMA (30 nM) for 1 d. (b) J774.1 cells and (c) mouse peritoneal macrophages were treated with LPS (2.5 μg/mL) or vehicle alone for 4 h. (d) PMA-pretreated HL-60 cells were stimulated with LPS (2.5 μg/mL), Pam3CSK4 (P3C4; 5 μg/mL), poly(I:C) (5 μg/mL), ODN1826 (ODN; 2.5 μM), or vehicle alone for 4 h. mRNA levels of USP2A, USP2B, and IL6 are normalized according to the level of HPRT-1 mRNA. Data presented as means ± SD of (a) five, (b) three, or (c and d) four samples. versus vehicle-treated control.

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