Research Article

Ubiquitin-Specific Protease 2 Modulates the Lipopolysaccharide-Elicited Expression of Proinflammatory Cytokines in Macrophage-like HL-60 Cells

Figure 4

Cytokine expression in peritoneal macrophages isolated from Usp2a Tg mice. Peritoneal macrophages were isolated from Usp2a Tg mice and their littermates, then stimulated with LPS (5 μg/mL) for 4 h. Transcript levels of Usp2 splice variants and cytokines were determined by qRT-PCR analysis. (a) Schematic summary of the comprehensive cytokine expression analysis in Usp2a Tg macrophages. (b) Expression data of nine cytokine transcripts whose level was attenuated by the overexpression of Usp2a. Data were normalized according to the HPRT-1 transcript level and expressed as means ± SD of five mice. versus vehicle-treated cells. § versus macrophages isolated from control mice.