Review Article
Contribution of In Vivo and Organotypic 3D Models to Understanding the Role of Macrophages and Neutrophils in the Pathogenesis of Psoriasis
Table 2
Observed features in 3D organotypic skin models.
(a) |
| 3D in vitro models | Components | Support | Observed features |
| Dezutter-Dambuyant et al. [102] | Fibroblasts + keratinocytes + endothelialized cells + CD34-positive cells | Solid scaffold of bovine collagen, chitosan and chondroitin 4-6 sulfate | Differentiation of interstitial dendritic dells | Bechetoille et al. [104] | Fibroblasts + dermal macrophages derived from monocytes | Solid scaffold of bovine collagen, chitosan and chondroitin 4-6 sulfate | Display phagocytosis and remain responsive to LPS | Chau et al. [103] | Fibroblasts + keratinocytes + dendritic cells | Nondegradable microfibre scaffolds and a cell-laden gel | Able to migrate and remain responsive to stimulation with skin sensitizers | Pageon et al. [106] | Fibroblasts + keratinocytes + monocytes | AGE-modified collagen lattices | Differentiation of CD14+ monocytes into dendritic cells and macrophages |
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(b) 3D models for psoriasis (without immune cells). |
| 3D in vitro models | Components | Support | Observed features |
| Barker et al. [111] | Fibroblasts + keratinocytes isolated from human lesional skin | Collagen gels | Psoriasis-like phenotype | Tjabringa et al. [98] | Healthy keratinocytes + de-epidermized dermis + cytokines (IL-1α, TNF-α, IL-6 and IL-22) | De-epidermized dermis | Psoriasis-like phenotype | Jean et al. [97] | Psoriatic fibroblasts + psoriatic keratinocytes | Fibroblast-derived dermal matrix | Psoriasis-like phenotype | van den Bogaard et al. [105] | Healthy keratinocytes + activated CD4-positive T cells and Th1/Th17-polarized T cells | Decellularized deepidermized dermis | Psoriasis-like phenotype |
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