Research Article
The Lymphotoxin β Receptor Is Essential for Upregulation of IFN-Induced Guanylate-Binding Proteins and Survival after Toxoplasma gondii Infection
Table 1
Primer and probe sequences for RT-PCR.
| | Target | Primers | Probe |
| | β-Actin | 5′TGACAGGATGCAGAAGGAGA
3′CGCTCAGGAGGAGCAATG | a106 | | mGBP1 | 5′CAGACTCCTGGAAAGGGACTC
3′CTTGGACCTGGAACATTCACTGAC | a41 | | mGBP2 | 5′TGAGTACCTGGAACATTCACTGAC
3′AGTCGCGGCTCATTAAAGC | a17 | | mGBP3 | 5′GGCTGAGGACTGTCCCTGT
3′CATGGTCCACTCGGAAGC | a21 | | mBGP4 | 5′GCCAAGATCAAGACCCTCAG
3′CCACGTAGGTTGTCACCAGA | a48 | | mGBP5 | 5′TCACTGAAGCTGAAGCAAGG
3′GCGTCAAAAACAAAGCATTTC | a48 | | mGBP6 | 5′ATATTTCAACATTTTTTGTTCCTTGT
3′GAAATGGGAGAAAAAATAAATGAAGC | FAM-AGTCATGTTCAATCTTCTCCCTCTTGTCC-DB | | mGBP7 | 5′GCAGAGAATCCGGTGCAG
3′TTTCCACTAGGCACACAGGA | a93 | | mGBP8 | 5′AAGAAGCTGAAGGAACAAAAGGC
3′GAAATGGGAGAAAAAATAAATGAAGC | FAM-TGTTTCAGTTGCTGTATCTCTCCGTCCA-TMR | | mGBP9 | 5′TTCCAAAACTTTCTCCAGTCACAGTA
3′GGCACGCTCCTCTGCAA | FAM-CCAGCAGTGAGGGCTCTATCTGCCT-TMR | | GTPBP1 | 5′GGTGCAGAGCAAAGATGATG
3′ATCTGGAATATCGGGCACAT | a75 | | IL-4 | 5′CATCGGCATTTTGAACGAG
3′CGAGCTCACTCTCTGTGGTG | a2 | | IL-12p40 | 5′GATTCAGACTCCAGGGGACA
3′TGGTTAGCTTCTGAGGACACATC | a27 | | iNOS | 5′CTTTGCCACGGACGAGAC
3′TCATTGTACTCTGAGGGCTGAC | a13 | | LTα | 5′TCCCTCAGAAGCACTTGACC
3′GAGTTCTGCTTGCTGGGGTA | a62 | | LTβ | 5′CCTGGTGACCCTGTTGTTG
3′TGCTCCTGAGCCAATGATCT | a76 | | IFNβ | 5′CAGGCAACCTTTAAGCATCAG
3′CCTTTGACCTTTCAAATGCAG | a95 |
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aNumbers identify probes obtained from the Roche Universal ProbeLibrary (Roche).
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