TSG-6 Downregulates IFN-Alpha and TNF-Alpha Expression by Suppressing IRF7 Phosphorylation in Human Plasmacytoid Dendritic Cells

Figure 6

The suppressive effect of TSG-6 was dependent on CD44. GEN 2.2 cells were treated with anti-CD44 blocking antibody at various concentrations as indicated or with control IgG (mouse IgG2b) for 45 min, followed by TSG-6 (1000 ng/mL) overnight, and stimulation with CpG-A (2 μM) (a) or R837 (10 ng/mL) (b) for 6 hours. Culture supernatants were collected for TNF-α and IFN-α quantification by ELISA. Graphs showing data from three independent experiments performed in duplicate. Error bars indicate SEM. One-Way ANOVA, , , and .

(a)

(b)