The Autophagy Level Is Increased in the Synovial Tissues of Patients with Active Rheumatoid Arthritis and Is Correlated with Disease Severity

Table 2

Primers used for real-time PCR.

Name	Sequence (5′–3′)	Annealing temp (°C)
BECN1	Sense: GGATGGTGTCTCTCGCAGAT	59.8
	Antisense: CAGTCTTCGGCTGAGGTTCT	59.8
LC3	Sense: ACCCTGAGTCTTCTCTTCAGGT	60.2
	Antisense: GTTGCGCTTCACAACTCAGG	60.0
Atg5	Sense: AGGCAACCTGACCAGAAACA	57.8
	Antisense: GAGGAAAGCAGAGGTGATGC	59.8
GADPH	Sense: CAGGAGGCATTGCTGATGAT	58.4
	Antisense: GAAGGCTGGGGCTCATTT	56.3

BECN1, beclin1 autophagy-related gene; LC3, microtubule-associated protein-light chain 3; Atg5, autophagy-related gene 5; GADPH, glyceraldehyde-3-phosphate dehydrogenase.