Taenia crassiceps Antigens Control Experimental Type 1 Diabetes by Inducing Alternatively Activated Macrophages

Figure 5

Exposure to TcES reduces T1D damage. Mice were injected with soluble antigen (TcS) or excreted/secreted antigen (TcES). Blood glucose levels were measured for 6 weeks after T1D induction. (a) Methodology diagram illustrating injection of T. crassiceps antigen (TcS and TcES). (b) Blood glucose of mice treated with different doses of TcS post T1D induction. (c) Glycemia of mice receiving constant and post T1D induction treatment with TcES. (d) Percentage of mice free of diabetes. Mice with glycemia greater than 200 mg/dl were considered diabetic, whereas those with levels of 200 mg/dl or lower were considered free of diabetes. Differences between STZ and STZ/TcES-4. (e) Histology of pancreas. Arrows represent insulitis and pancreatic islet damage. (f) Insulitis score. Noninfiltrated: without damage. Peri-insulitis: infiltration only at the periphery of the islets. Insulitis 20%: infiltration of ≥20% or more but less of 40%. Insulitis 40%: infiltration of ≥40% of islets. Data are representative of 2 independent experiments. mice per group. For comparisons between two groups, the Mann–Whitney U test was applied to test differences with nonparametric data. Experiments with multiple groups were tested by the Kruskal-Wallis test followed by Dunn’s multiple comparison test. The data shown are the mean ± SEM. .

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