A Proinflammatory Effect of the β-Glucan from Pleurotus cornucopiae Mushroom on Macrophage Action

Figure 6

Combination effect of guanyl acid/vitamin D₂ with PCPS on proinflammatory cytokines secretion from the stimulated macrophages THP-1. Human monocyte cell line THP-1 was differentiated into macrophages by PMA (160 nM) for 48 hrs., and then they were stimulated with 1 μg/ml of the 450 kD polysaccharide (PCPS) purified from a hot water extract of P. citrinopileatus fruiting bodies plus the different concentrations of 5′-GMP-Na (Figure 6(a)) or ergocalciferol as vitamin D₂ (Figure 6(b)). After 24 hours, the secretion of TNF in the cell supernatant was determined by ELISA. The data shown are the average of 3 independent experiments. The depicted error bars represent the SEM of these 3 experiments. For statistical analysis, a one-way ANOVA followed by the Dunnett’s t-test was used; when compared to the RPMI medium control.

(a)

(b)