Research Article

Human β-Defensin 3 Reduces TNF-α-Induced Inflammation and Monocyte Adhesion in Human Umbilical Vein Endothelial Cells

Figure 4

Effects of hBD3 on TNF-α-induced adhesion to THP-1 cells in HUVECs. HUVECs were stimulated with TNF-α (40 ng/mL) and the indicated concentrations of hBD3 for 24 h. After stimulation, HUVECs were cocultured with calcein-AM-labeled THP-1 cells for 30 min. (a) Images were captured using a fluorescence microscope (original magnification: 100x). (b) Adherent cells were measured using a microplate reader at excitation and emission wavelengths of 490 nm and 515 nm, respectively. The results were expressed as the mean ± SD, . compared with the control group. compared with TNF-α group.
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