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Figure 3: Effects of iron on mRNA expression in M1 macrophage. (a) IL-6, (b) IL-1β, (c) TNF-α, and (d) iNOS mRNA expression were assessed by real-time PCR. Gene expression is represented as fold-change compared to unstimulated macrophages. Data are mean ± SD for three independent experiments. (uns: unstimulated macrophages; ).