Davallia mariesii Moore Improves FcεRI-Mediated Allergic Responses in the Rat Basophilic Leukemia Mast Cell Line RBL-2H3 and Passive Cutaneous Anaphylaxis in Mice
Effect of Drynaria rhizome extract (DRE) on the expression of Src family kinase genes in RBL-2H3 mast cells. The cells were treated with DRE for 1 h and then treated with DNP-HSA (0.1 μg/mL) for 30 min. (a) Histamine levels in the culture medium of IgE-sensitized RBL-2H3 mast cells treated with DRE. (b) Immunoblot analysis performed with anti-p-Syk, anti-p-Lyn, anti-p-Fyn, anti-p-PLCγ1, anti-p-ERK, and anti-p-Akt antibodies. α-Tubulin was used as protein loading control. Results are expressed as means ± SE of at least five independent experimental results that were tested by analysis of variance with Bonferroni’s post-hoc testing; #, the control group versus the DNP-HSA group; , , and , the DNP-HSA group versus the DRE and Dex treatment-group.