HIF1α-Induced Glycolysis Metabolism Is Essential to the Activation of Inflammatory Macrophages

Figure 1

HIF1α induced M1 polarization of macrophages. (a) The relative mRNA level of HIF1α in the BMDMs and peritoneal macrophages of WT mice and Lysm Hif1α lsl mice. (b) The relative mRNA levels of M1 and M2 markers in the peritoneal macrophages isolated from WT mice and Lysm HIF1α lsl mice for 6 h. (c) The relative mRNA levels of M1 and M2 markers in the peritoneal macrophages isolated from WT mice and Lysm HIF1α lsl mice for 24 h. (d) The relative mRNA levels of M1 and M2 markers in the BMDMs isolated from the WT mice and Lysm HIF1α lsl mice with the treatment of LPS for 48 h. For each gene, mRNA level was normalized to the level of 18S rRNA expression. Statistical comparisons were made using two-tailed Student’s t-test (a, b, c, and d). and , compared with WT mice. All values were presented as mean ± SEM for independent experiments in each group.

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