Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes
Effect of A. baumannii-LPS on adipokine expression in 3T3-L1 adipocytes. Differentiated adipocytes were incubated for 24 h with 100 ng/mL A. baumannii-LPS (ATCC 19606), 100 ng/mL E. coli-LPS (O111:B4), or saline and subjected to real-time PCR analysis to determine the mRNA levels of several adipokines, including lipocalin-2, FABP4, leptin, and adiponectin. Adipokine expression is presented as the ratio of viable adipokine expression in adipocytes treated with the LPS from each of the bacterial strains versus the adipocytes treated with saline. All experiments were performed in triplicates, and the results are shown as mean ± SD. ; (compared to adipocytes without LPS treatment).