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Figure 6: Detection of mRNA (a) and the number of positive cells of pro- (IL-1β, TNF-α, and 5-LOX) versus anti-inflammatory (15-LOX, FPR2, and IL-10) mediators (b and c) in patients with osteoarthritis (OA). Quantification of mRNA of pro- versus anti-inflammatory mediators shows that anti-inflammatory mediator expression was more prominent than inflammatory mediators in OA synovium. Relative to control (, one-way ANOVA followed by Tukey’s test). (b) Quantitative analysis of immunofluorescent microscopy pro- versus anti-inflammatory mediators shows that the number of cells expressing anti-inflammatory mediators was more prominent than those containing proinflammatory mediators in OA synovium; the asterisks denote significant differences (, one-way ANOVA followed by Dunn’s test). Data are shown as means ± SEM. (c) Confocal microscopy of pro- (red fluorescence; (A) and (B)) and anti-inflammatory mediators (green fluorescence; (C) and (D)) double immunofluorescence in OA synovium. Note that anti-inflammatory mediator expression was more prominent than proinflammatory mediators in OA synovium. Bar = 40 μm.