Regular Voluntary Exercise Potentiates Interleukin-1β and Interleukin-18 Secretion by Increasing Caspase-1 Expression in Murine Macrophages

Figure 6

Effects of regular voluntary exercise (VE) on the activation of intracellular signaling downstream of TLR4 in macrophages stimulated with or without lipopolysaccharide (LPS). Peritoneal-exudate macrophages isolated from the sedentary control (SC) and VE mice were cultured for 20 min in the presence or absence of 100 ng/mL LPS. The protein levels of inhibitor of κB (IκB)α (a) and phosphorylation levels of IκB kinase (IKK)β (b), p54 (c), p46 (d), and p38 (e) were analyzed by western blotting (upper panel). The protein levels are presented as ratios relative to the levels of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (lower panel), whereas the phosphorylation levels are presented as ratios relative to the respective protein levels (lower panel). Mean ± standard error of the mean (SEM; ) values are provided. (by one-way analysis of variance [ANOVA] and the Bonferroni test).

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