Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Figure 4

The impact of CPS on the expression of MyD88-independent TLR signaling pathway in ALI cultures of sheep bronchial epithelial cells. 4-week old ALI cultures of sheep bronchial epithelial cells were exposed to the indicated conditions for 48 h. (a) The expression of several key components of MyD88-independent signaling cascade, including the TRAF3, TBK1, and TRAM transcripts were determined by qRT-PCR assays. A significantly more abundant TRAF3 and TRAM2 mRNA was detected in ALI cultures treated with CPS/M. ovipneumoniae, in comparison with uninfected controls, while no evident alternations of TBK1 and TRAM1 expression were observed. (b) Expression levels of MyD88-independent signaling-associated proteins. Results showed that the expression of TRIF, TRAF3, and TBK1 were remarkably upregulated after treatments of CPS/M. ovipneumoniae. The immunoblots showed here were representative of three independent experiments with similar results. (c) Densitometric values are shown for TRIF, TRAF3, and TBK1 proteins over β-actin. Values are mean ± SD for at least three independent experiments performed in triplicate. and versus that of the control. Compared between indicated groups, ^▲▲.

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