Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells

Figure 6

Induction of proinflammatory mediators and cytokines in the ALI cultures of sheep bronchial epithelial cells upon CPS stimulation. 4-week old ALI cultures of sheep bronchial epithelial cells were exposed to the indicated conditions for 48 h before the samples were harvested for analysis. (a–c) The mRNA expression of cytokines and chemokines including IL1α, IL1β, IL4, IL6, IL8, IL10, IL12A, IL12B, IL13, IL17A, IL17B, IL18, IL23A, IL33, TNFα, IFNβ, and TGFβ were determined by qRT-PCR assays. Compared to the control, all tested transcripts showed a significant increase after a treatment of CPS or M. ovipneumoniae. (d) Effect of CPS or MO on TNFα, IL1β, IL6, IL8, and IL10 production in the supernatant of ALI cultures were determined by ELISA. Results showed the protein expression of TNFα, IL1β, IL8, and IL10 was significantly elevated in cells upon CPS/M. ovipneumoniae stimulation, in comparison with uninfected controls, while no remarkable alteration of IL6 expression was observed. These bar graphs shown here are representative of three independent experiments with similar results. and versus that of the control. Compared between indicated groups, ^▲ and ^▲▲.

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