Mediators of Inflammation / 2018 / Article / Tab 1 / Research Article
Folic Acid Improves the Inflammatory Response in LPS-Activated THP-1 Macrophages Table 1 CpG methylation levels (as percentage) in
IL1B ,
TNF ,
IL18 , and
SERPINE1 genes after the incubation of THP-1 monocytes with folic acid, choline, vitamin B
12 , and the methyl donor mixture, measured by MassARRAY. Unpaired Student’s
t -test was used to compare each CpG with the control group.
value < 0.05;
value < 0.01; and
value < 0.001.
Control Folic acid Choline Vitamin B12 Methyl donor mixture IL1B CpG_1 6.88 ± 3.97 15.5 ± 6.14 11.4 ± 1.55 9.38 ± 0.95 14.0 ± 3.11 CpG_2 94.9 ± 3.14 96.2 ± 1.66 94.8 ± 2.06 9.76 ± 0.85 96.9 ± 0.85 CpG_3 2.88 ± 2.09 1.75 ± 1.26 5.75 ± 1.77 3.75 ± 3.89 3.12 ± 3.75 CpG_4 2.75 ± 2.06 1.88 ± 1.79 1.25 ± 1.19 1.75 ± 0.96 3.75 ± 1.55 CpG_5 2.12 ± 2.49 6.50 ± 4.06 1.25 ± 0.64 3.25 ± 1.55 10.0 ± 1.13 CpG_6 0.75 ± 0.50 7.12 ± 6.14 9.50 ± 0.58 9.25 ± 2.33 0.88 ± 0.48 TNF CpG_1 98.2 ± 1.32 96.1 ± 2.78 98.0 ± 1.47 95.2 ± 2.72 95.8 ± 3.07 CpG_2 67.8 ± 3.95 64.8 ± 7.59 65.8 ± 4.48 69.9 ± 1.93 63.4 ± 6.46 CpG_3 48.1 ± 12.6 48.6 ± 8.53 51.2 ± 10.3 47.9 ± 4.71 45.0 ± 4.65 CpG_4.5.6 19.9 ± 6.76 15.2 ± 4.41 17.4 ± 3.09 19.8 ± 0.87 17.8 ± 1.79 CpG_8 33.9 ± 3.49 29.6 ± 4.37 31.8 ± 3.93 34.2 ± 2.53 34.0 ± 3.24 IL18 CpG_1 12.5 ± 17.4 2.0 ± 0.5 2.12 ± 0.85 1.25 ± 1.32 2.5 ± 2.0 CpG_2 6.88 ± 1.55 6.0 ± 1.22 7.38 ± 2.06 9.50 ± 1.0 10.1 ± 2.25 CpG_3 0.67 ± 0.29 2.0 ± 0.82 0.62 ± 0.25 1.38 ± 0.75 0.88 ± 0.75 CpG_4 11.0 ± 2.04 8.38 ± 4.09 7.75 ± 2.22 8.0 ± 1.91 7.25 ± 1.85 CpG_5 19.0 ± 3.19 13.2 ± 2.10 15.8 ± 2.90 16.6 ± 1.11 15.0 ± 2.16 SERPINE1 CpG_1 2.62 ± 0.63 22.0 ± 13.7 10.8 ± 14.2 17.0 ± 16.1 56.2 ± 5.14 CpG_2 33.5 ± 3.39 63.0 ± 12.5 50.8 ± 7.09 65.0 ± 5.40 45.4 ± 8.68 CpG_3.4 38.5 ± 9.81 91.1 ± 8.23 79.5 ± 7.99 85.2 ± 10.9 62.6 ± 24.0 CpG_6 100 ± 0.00 89.4 ± 4.09 90.9 ± 11.3 87.1 ± 10.2 96.6 ± 5.49 CpG_7 2.88 ± 1.60 32.1 ± 15.4 13.9 ± 7.97 28.2 ± 23.1 15.8 ± 15.8 CpG_8 95.1 ± 2.62 91.5 ± 6.77 92.6 ± 6.74 93.1 ± 2.66 94.9 ± 2.06 CpG_9 33.5 ± 3.39 63.0 ± 12.5 50.8 ± 7.09 65.0 ± 5.40 45.4 ± 8.68 CpG_10 97.2 ± 2.59 95.0 ± 3.03 96.6 ± 1.60 88.5 ± 19.7 96.9 ± 2.46 CpG_11 97.5 ± 2.91 98.2 ± 2.36 97.1 ± 2.69 98.1 ± 0.75 98.5 ± 2.68 CpG_12 94.0 ± 7.22 95.0 ± 2.42 94.2. ± 5.52 96.0 ± 3.58 92.4 ± 6.26
