Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells

Figure 1

Induction and detection of SelS in LDLR-KO mice and TNF-α-treated HUVECs. (a) Representative images of SelS expression detected by immunohistochemistry, and representative images captured at 10x and 40x magnifications. (b) Mean density of SelS analyzed in different groups with the semiquantitative method using the Image Pro Plus 6.0 software. The sections from six mice of each group were observed and three different fields of each slice were randomly selected. (c) Expression of SelS tested in HUVECs at different time points with TNF-α (10 ng/ml) stimulation. (d) SelS expression determined in HUVECs with different concentrations of TNF-α treatment (for 6 h of incubation). The results are representative of triplicate independent experiments and are presented as mean ± SD, (). versus control. LDLR: low-density lipoprotein receptor; KO: knockout; HUVECs: human umbilical vein endothelial cells; RC: regular chow; HFD: high-fat diet.

(a)

(b)

(c)

(d)