Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells

Figure 4

Effect of SelS on the levels of ET-1 and ROS in TNF-α-treated HUVECs. The mRNA levels of ET-1 detected in HUVECs transfected with SelS plasmid (a) or SelS siRNA (b) after 10 ng/ml TNF-α induction. (c) The levels of cellular ROS measured in different groups with 10 ng/ml TNF-α treatment using fluorescent probe method and (d) the corresponding fluorescent mean intensity evaluated by the Image Pro Plus 6.0 software. The cells were transfected with pcDNA3.1-SelS recombinant plasmid or SelS siRNAs for 30 h. The levels of ET-1 and ROS were tested after 6 h TNF-α treatment. The results are representative of triplicate independent experiments and are presented as mean ± SD, (). versus control; versus empty vector; versus negative siRNA. ROS: reactive oxygen species; ET-1: endothelin-1; E-vector: empty vector; Pc-SelS: pcDNA3.1-SelS plasmid; Neg.RNA: negative siRNA.

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