Selenoprotein S Attenuates Tumor Necrosis Factor-α-Induced Dysfunction in Endothelial Cells

Figure 5

Effect of SelS on THP-1 cells adhesion and adhesion factors. (a) Adhesion of THP-1 cells to HUVECs shown by the fluorescent probe method and (b) the corresponding fluorescence intensity analyzed using Image Pro Plus 6.0 software. The production of ICAM-1 (c) and VCAM-1 (d) detected in the supernatant of cultures with 10 ng/ml TNF-α treatment by ELISA. The mRNA levels of ICAM-1 estimated in 10 ng/ml TNF-α-induced cells after transfection with SelS plasmid (e) or SelS siRNA (f). The mRNA levels of VCAM-1 determined in SelS plasmid (g) or SelS siRNA (h) transfected cells after 10 ng/ml TNF-α induction (for 6 h). The cells were transfected with pcDNA3.1-SelS recombinant plasmid or SelS siRNAs for 30 h. The results are representative of triplicate independent experiments and are presented as mean ± SD, (). versus control; versus empty vector; versus negative siRNA. ICAM-1: intercellular adhesion molecule-1; VCAM-1: vascular cell adhesion molecule-1; E-vector: empty vector; Pc-SelS: pcDNA3.1-SelS plasmid; Neg.RNA: negative siRNA.

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