Reduced NLRP3 Gene Expression Limits the IL-1β Cleavage via Inflammasome in Monocytes from Severely Injured Trauma Patients

Figure 3

Transfecting CD14⁺ monocytes with NLRP3 recovers their functionality. CD14⁺ monocytes were isolated either from healthy volunteers (HV) or from trauma patients (TP) after the admission. Subsequently, cells were transfected with the NLRP3 expression plasmid or with plasmid control (ctrl) before stimulation with lipopolysaccharide (LPS). In the supernatants, secretion of IL-1β was determined by ELISA. Data are shown as mean ± SEM. versus indicated.