Evidence That ADAM17 Mediates the Protective Action of CGRP against Angiotensin II-Induced Inflammation in Vascular Smooth Muscle Cells

Figure 1

CGRP dampened Ang II-induced inflammation in vascular smooth muscle cells. After pretreating with 10 nmol/l CGRP or/and 50 nmol/l CGRP8-37 for 30 minutes, vascular smooth muscle cells were stimulated with 100 nmol/l Ang II for 24 hours, then the medium was collected to assay for the concentration of IL-1β, IL-6, and TNF-α by ELISA, while the cells were adopted to measure the mRNA levels of IL-1β, IL-6, and TNF-α. (a–c) the protein levels of IL-1β (a), IL-6 (b), and TNF-α (c), –5 independent experiments. (d) The mRNA levels of IL-1β, IL-6, and TNF-α, independent experiments. Ang II represents angiotensin II. versus control, versus control, versus Ang II, and versus Ang II + CGRP.

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