Contribution of Neurons and Glial Cells to Complement-Mediated Synapse Removal during Development, Aging and in Alzheimer’s Disease

Table 2

Cellular localization of complement components in adulthood and aging.

Cell type	Localization	Experimental model	Method	Ref.
C1q
Neuron	Synaptic puncta	In vivo: C57BL/6 mice; Human tissue	IHC	[32]
Microglia	Cellular	In vitro: mice primary culture; In vivo: C57BL/6, C1qa deletion mice	qRT-PCR, IF, IHC, WB	[30–33]
Astrocyte	Exosomes	Human plasma	Immunoassay	[34]
C3
Microglia	Cellular	In vitro: mice primary cultures	qRT-PCR, IF, ICC, WB	[30, 31, 35]
Astrocyte	Cellular	In vitro: mice primary cultures	qPCR, IF, WB, RNA-seq	[30, 35, 36]
	Exosomes	Human plasma	Immunoassay	[34]
CR3
Microglia	—	In vitro: mice primary culture	qRT-PCR, IF	[31]
C3aR
Neuron	Cellular	In vitro: cultured neural stem cells, rat; In vivo: WT mice and rat	In situ hybridization, IF	[41, 42]
Microglia	Cellular	In vivo: WT mice; Human tissue	In situ hybridization, IF, IMC	[37–39]
Astrocyte	Cellular	In vitro: CB193 cell line, human astrocyte cultures	IF, RT-PCR, IP	[39, 40]

qRT-PCR: semiquantitative PCR; ICC: immunocytochemistry; IHC: immunohistochemistry; WB: Western blot; IF: immunofluorescence; FACS: fluorescence-activated cell sorting; IP: immunoprecipitation.