A Snake Venom-Secreted Phospholipase A₂ Induces Foam Cell Formation Depending on the Activation of Factors Involved in Lipid Homeostasis

Figure 7

MT-III-induced CD36 protein expression is dependent on PPAR-γ and PPAR-β/δ activation. Peritoneal macrophages were incubated with GW9662 (10 μM) or GSK0660 (10 μM) compounds or vehicle (<1% DMSO/RPMI) for 1 h and then with MT-III (0.4 μM) for 6 h. Western blotting and densitometric analysis of CD36 receptor and β-actin (loading control) in macrophage extracts. The densities (in arbitrary units) were normalized with those of β-actin. Results are expressed as mean ± SEM from three experiments. compared with controls; compared with vehicle-treated MT-III-stimulated cells.