Huanglian-Wendan Decoction Inhibits NF-κB/NLRP3 Inflammasome Activation in Liver and Brain of Rats Exposed to Chronic Unpredictable Mild Stress

Figure 5

Effects of Huanglian-Wendan decoction on NF-κB and NLRP3 inflammasome activation in the hippocampus of CUMS rats. (a) The nuclear entry of NF-κB was quantitated by the nuclear/cytoplasmic ratio of NF-κB protein levels normalized by lamin A/C or GAPDH, respectively. (b) The relative protein levels of p-NF-κB were normalized to NF-κB. (c) The relative protein levels of p-IκBα were normalized to IκBα. (d) The relative protein levels of NLRP3 were normalized to β-actin. (e) The relative protein levels of ASC were normalized to β-actin. (f) The relative protein levels of mature Caspase-1 were normalized to β-actin. (g) The relative protein levels of mature IL-1β were normalized to pro-IL-1β. Protein levels of p-NF-κB, NF-κB, p-IκBα, IκBα, NLRP3, ASC, mature Caspase-1, mature IL-1β, pro-IL-1β, and β-actin were analyzed by the Western blot method. The data were expressed as fold of control values. All data were expressed as (). and compared with the non-CUMS group; , , and compared with the CUMS group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)