Mediators of Inflammation / 2018 / Article / Fig 1

Research Article

Placental Ras Regulates Inflammation Associated with Maternal Obesity

Figure 1

Placental Ras expression with maternal obesity and with exposure to inflammation. Human placenta was obtained from lean and obese women at the time of term Caesarean section. (a) Ras protein expression was assessed by Western blot and normalised to β-actin ( patients per group). The average densitometry of the Ras doublet band was determined. A representative Western blot from 6 patients is shown. (b) Ras GTPase activity was measured in the placenta of lean and obese women ( per group). (a, b) For all data, the fold change was calculated relative to the lean group. Data are displayed as mean ± SEM. vs. lean (Student’s t-test). (c, d) Human placenta was incubated with 1 μg/ml LPS, 10 ng/ml TNF-α, or 1 ng/ml IL-1β for 24 h ( patients per group). (c) Ras protein expression was analysed by Western blotting and normalised to β-actin. The fold change was calculated relative to basal. (d) Ras GTPase activity was measured in the placenta exposed to LPS, TNF-α, and IL-1β. (c, d) The fold change was calculated relative to basal. Data are displayed as mean ± SEM. vs. basal (paired Student’s t-test). A representative Western blot from 1 patient is shown.
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