The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling

Figure 3

Effects of PSS on the inflammatory response in ConA-induced liver injury in mice. (a) The levels of serum TNF-α and IL-6 measured with ELISA were reduced by L-THP pretreatment in mice at doses of 20 mg/kg and 40 mg/kg. Data are presented as means ± SD (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, and for L-THP (40) + ConA versus ConA). (b) qRT-PCR was used to detect the mRNA expression of TNF-α and IL-6 in each group, and the results were expressed as the mean ± SD (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, and for L-THP (40) + ConA versus ConA). (c) Western Blot analysis was used to detect the protein expression of TNF-α and IL-6. Data were expressed as the mean ± SD (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, for L-THP (40) + ConA versus ConA). (d) The expression of TNF-α and IL-6 in liver tissue at 8 h was detected by immunohistochemical staining (Original magnification, ×200) (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, and for L-THP (40) + ConA versus ConA).

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