The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling

Figure 4

Effects of L-THP pretreatment on apoptosis and autophagy on ConA-induced liver injury in mice. (a) The mRNA expression of Bax, Bcl-2, caspase 9, Beclin-1, and LC3 was detected by qRT-PCR. Data were expressed as the mean ± SD (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, and for L-THP (40) + ConA versus ConA). (b) The protein expression of Bax, Bcl-2, caspase 3, caspase 9, Beclin-1, and LC3 was detected by Western Blotting. Data were expressed as the mean ± SD (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, and for L-THP (40) + ConA versus ConA). (c) Immunohistochemistry staining (×200) showed the expression of Bax, Bcl-2, Beclin-1, and LC3 proteins in liver tissues at 8 h. The positive areas and total area were measured using Image-Pro Plus. Data were expressed as the mean ± SD (, ⁺ for NC versus ConA, ^# for L-THP (20) + ConA versus ConA, and for L-THP (40) + ConA versus ConA).

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