Photochemical Treatment of Drosophila APCs Can Eliminate Associated Viruses and Maintain the APC Function for Generating Antigen-Specific CTLs Ex Vivo

Figure 6

The effect of UVDEX-treated APCs and CpG on the generation of antigen-specific CTLs. CD8 T cells were purified from one pooled B6 spleen and lymph node cells using anti-mouse CD8 beads by AutoMACS (Miltenyibiotech), and three mice were used for the experiment. The purified CD8 T cells were activated on 24-well plates with OVA-peptide-loaded and psoralen UV-treated or nontreated APCs (Fly/K^b/B7.1/ICAM) or nontreated APCs in the presence of 5 μM CpG oligo or 5 μM control oligo (GpC oligo). IL-2 was added at day 3 and day 5, and cells were split at day 7. At day 9, cells were collected and stained with OVA-specific tetramer-PE or D^b-GagL-specific control tetramer and anti-mouse CD8 mAb-FITC at room temperature for 30 min. FACS analysis of OVA-specific CTLs from CD8-positive cells.