Photochemical Treatment of Drosophila APCs Can Eliminate Associated Viruses and Maintain the APC Function for Generating Antigen-Specific CTLs Ex Vivo

Figure 7

MyD88-dependent enhancement of the activation of antigen-specific CTLs by UVADEX-treated APC. CD8 T cells were purified from one pooled B6 spleen and lymph node cells or one MyD88−/− mouse spleen and lymph node cells by AutoMACS (Miltenyibiotech), respectively. Three B6 mice and three MyD88−/− mice were used for the experiment. The purified CD8 T cells were activated on 24-well plates with OVA-peptide loaded and UVADEX-treated APCs (Fly/K^b/B7.1/ICAM) or UVDEX nontreated APCs (control for psoralen treatment) or nontreated APCs in the presence of 5 μM CpG oligo or 5 μM control oligo (GpC oligo). IL-2 was added at day 3 and day 5, and cells were split at day 7. At day 9, cells were collected and stained with OVA-specific tetramer-PE and or D^b-GagL-specific control tetramer and anti-mouse CD8 mAb-FITC at room temperature for 30 min. FACS analysis of OVA-specific CTLs from CD8-positive cells.