[Retracted] The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages

Figure 2

Effects of Shinbaro3 on NO production in LPS-stimulated RAW 264.7 macrophage cells. (a) RAW 264.7 cells were stimulated with LPS (1 μg/mL) for 20 h in the absence or presence of Shinbaro3 (after hydrolysis) or H. procumbens extract (before hydrolysis) (200, 400, 800, or 1000 μg/mL). The nitrite concentration in the supernatant was detected via the Griess reaction. (b) Cell viability upon Shinbaro3 or H. procumbens treatment for 20 h was evaluated using the MTT assay, as described in Materials and Methods. (c) Morphological changes produced by Shinbaro3 in LPS-stimulated macrophages, photographed with an inverted phase-contrast microscope. Data are presented as the mean ± SD (). , , and versus LPS treatment alone.

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