[Retracted] The Anti-Inflammatory Effects of Shinbaro3 Is Mediated by Downregulation of the TLR4 Signalling Pathway in LPS-Stimulated RAW 264.7 Macrophages

Figure 6

Effects of Shinbaro3 on the IRF3/STAT1 signalling pathway in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with LPS (1 μg/mL) and Shinbaro3 (150, 300, and 450 μg/mL) for 4 h. The expression levels of (a) IRF3, STAT1, JAK1, and their phosphorylated forms were detected with specific antibodies. β-Actin was used as an internal control. The data are representative of three separate experiments. (b) Protein expression of INF-β was assessed in RAW 264.7 cells under the above condition. (c) RAW 264.7 cells were treated with LPS (1 μg/mL) and Shinbaro3 (150, 300, and 450 μg/mL) for 6 h. Expression of IFN-β was investigated with real-time RT-PCR as described in Materials and Methods. (d) RAW 264.7 cells were stimulated with Shinbaro3 (150, 300, and 450 μg/mL) in presence of IFN-β (100 U/mL) for 4 h. Protein expression of JAK1 and STAT1 and their phosphorylated forms were detected. The data are expressed as the mean ± SD (). , , and versus LPS treatment alone.

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