A Profibrotic Phenotype in Naïve and in Fibrotic Lung Myofibroblasts Is Governed by Modulations in Thy-1 Expression and Activation

Figure 7

Decrease in Thy1 fibroblast expression during myofibroblast differentiation. Lung myofibroblasts in primary culture were stimulated for 72 h with TGFβ (2 ng/ml). (a) Cells were fixed and stained with anti-α-SMA antibody (red) and PI (green) and analyzed by confocal microscope. (b) Cells were stained using PE-conjugated anti-Thy1 and analyzed by FACS. (c) Graphical presentation of results from (b).

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