Research Article

Osthole Protects against Acute Lung Injury by Suppressing NF-κB-Dependent Inflammation

Figure 2

Osthole inhibits nuclear translocation of NF-κB. The effects of osthole on LPS-activated NF-κB were determined from MPMs pretreated with the indicated concentrations of osthole for 1 h and stimulated with LPS (0.5 μg/mL) for 0.5 h (a–b) or 1 h (c). (a) Representative Western blot analysis of cell lysates for IκB-α; GAPDH as loading control; . (b) Representative Western blot analysis of cytoplasmic and nuclear cell fractions for the p65 subunit of NF-κB (C-P65 and N-P65, resp.); GAPDH and lamin B are respective loading controls; . (c) For immunofluorescence evaluation of nuclear translocation of the p65 subunit, the osthole-pretreated MPMs were stimulated with LPS (0.5 μg/mL) for 1 h; p65 (red) and DAPI nuclear stain (blue), , micron .
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