Research Article

Osthole Protects against Acute Lung Injury by Suppressing NF-κB-Dependent Inflammation

Figure 6

Osthole suppresses NF-κB signaling in vivo. ALI was induced as described (Materials and Methods). Briefly, C57BL/6 mice were pretreated for 30 min with 20 mg/kg or 40 mg/kg osthole via ip injection, followed by intratracheal injection of 5 mg/kg LPS, and lungs were sampled 6 h later for Western blot analyses. (a) Representative Western blot detecting for IκB-α; GAPDH as loading control. (b) Densitometric quantification of blot in (a); values are reported as relative to control (Ctrl); . The bands were quantified by ImageJ and normalized to loading controls. (c) Representative Western blot analysis of the p65 subunit of NF-κB from cytoplasmic and nuclear cell fractions (C-p65 and N-p65, resp.); GAPDH and lamin B as respective loading controls. Densitometric quantification of (d) N-p65 and (e) C-p65 from (c); values are reported as ; . The bands were quantified by ImageJ and normalized to loading controls. ns = not significant; , versus control (Ctrl). , versus vehicle.
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