IL-17 Receptor Signaling Negatively Regulates the Development of Tubulointerstitial Fibrosis in the Kidney
Overexpression of IL-17 reduced TF development in the WT mice. WT mice (–8) were either injected with adenovirus overexpressing IL-17 (Ad-IL-17) or control vector (Ad-ctrl) (109 pfu) 72 h prior to performing UUO surgery. The TF development was evaluated at day 7 post-surgery by (a) Masson’s trichrome staining of serial sections from UUO kidneys. Photomicrographs are representative of 2 independent experiments. Original magnification: 10x. (b) The total collagen content in the kidneys was evaluated by hydroxyproline assay. (c) WT mice () were treated with a cocktail of antibiotics (Abx) in drinking water starting day 7 (relative to UUO surgery) and then throughout the experiment. Control mice (no Abx treatment) received drinking water only. At day 7 post-surgery, total collagen content in the kidney was evaluated by hydroxyproline assay. In the scattered plots, each dot represents individual mouse and error bars indicate . The data are pooled from two independent experiments. value ≤0.05 (), ≤0.01 (), and ≤0.0001 (). ns: not significant.
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